구강 바이오필름에 대한 피톤치드의 항균능력
Antibacterial activity of phytoncide on oral biofilm
김민정, 이명구, 이준행, 전열매, 유현준,
소속 상세정보
김민정 ( Kim Min-Jeong ) - Dankook University College of Health Science Department of Oral Health
이명구 ( Lee Myung-Gu ) - Konkuk University Department of Biomedical Science and Engineering
이준행 ( Lee Joon-Haeng ) - Dankook University College of Dentistry Department of Pediatric Dentistry
전열매 ( Jeon Yeol-Mae ) - Dankook University College of Dentistry Department of Preventive Dentistry
유현준 ( Yoo Hyun-Jun ) - Dankook University College of Dentistry Department of Preventive Dentistry
Abstract
Objectives: This study aimed to evaluate the antibacterial activity of phytoncide on oral biofilm.
Methods: Antibacterial activity of phytoncide was examined on the bacteria Streptococcus mutans, Aggregatibacter actinomycetemcomitans, Enterococcus faecalis, Porphyromonas gingivalis, and oral biofilm. After mixing the phytoncide, S. mutans, A. actinomycetemcomitans, E. faecalis, and P. gingivalis with a culture alone turbid and mixed, and they were then incubated at 37°C under anaerobic conditions and cultured. Following incubation, a microplate reader was used to measure the absorbance and observe the amount of bacteria. In addition, non-polarized saliva containing bacteria was grown for 72 h under anaerobic conditions for the determination of biofilm biomass and bacterial numbers. Various concentrations of phytoncide were added to the saliva biofilm. Statistical significance tests were conducted using the Mann-Whitney test and SPSS 24.0.
Results: S. mutans, A. actinomycetemcomitans, E. faecalis, P. gingivalis, and antibacterial effects were shown as phytoncide concentrations increased in oral biofilm. Observing the growth of bacteria using phase difference microscopes showed that the number of bacteria decreases as the phytoncide concentration increased. Also, the formation of biofilm in the saliva decreased compared to the control group.
Conclusions: Based on the experimental results of phytoncide on oral biofilms causing oral cavity formation in the saliva decreased compared to that in the control group. Phytoncide showed antibiosis against oral biofilms when it remained inside the mouth for above certain concentrations. Accordingly, using phytoncide as a clinical method for preventing oral disease is deemed to be effective.
키워드
Aggregatibacter actinomycetemcomitans; Enterococcus faecalis; Oral biofilm, Phytoncide, Porphyromonas gingivalis, Streptococcus mutans
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